Please use this identifier to cite or link to this item:
https://cris.library.msu.ac.zw//handle/11408/4160
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shoushtari, Maryam Sharif | |
dc.contributor.author | Majd, Ahmad | |
dc.contributor.author | Assarehzadegan, Mohammad-Ali | |
dc.contributor.author | Fanuel, Songwe | |
dc.contributor.author | Moin, Mostafa | |
dc.contributor.author | Nejadsattari, Taher | |
dc.contributor.author | Shoormasti, Raheleh Shokouhi | |
dc.contributor.author | Badalzadeh, Mohsen | |
dc.contributor.author | Tajik, Shaghayegh | |
dc.contributor.author | Fazlollahi, Mohammad Reza | |
dc.contributor.author | Tayebi, Behnoosh | |
dc.contributor.author | Pourpak, Zahra | |
dc.contributor.author | Kardar, Gholam Ali | |
dc.date.accessioned | 2021-05-11T10:32:19Z | |
dc.date.available | 2021-05-11T10:32:19Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 21970378 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs40629-020-00129-3 | |
dc.identifier.uri | http://hdl.handle.net/11408/4160 | |
dc.description.abstract | Background Fraxinus excelsior (Ash) is a common tree and is important cause of winter–spring pollinosis in many temperate regions in the world. In this study, a high molecular weight allergen from ash pollen was identified. Methods In all, 20 individuals allergic to ash participated in the study. Characterization and immunoreactivity of ash pollen proteins was performed using sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE), two-dimensional (2D) gel electrophoresis and immunoblotting. Results Immunoglobulin E (IgE)-binding proteins with apparent molecular mass ranging from 9 to 110 kDa were detected in ash pollen extract. Serum IgE of 7 (35%) patients reacted with a 53-kDa protein band. Analysis of 2D immunoblots showed several IgE-binding proteins. Moreover, mass spectrometry analysis indicated that the 53-kDa allergen was homologous to calreticulin. Discussion We defined a novel allergen from F. excelsior pollen with a molecular weight of about 53 kDa. This allergen could be considered as an important high molecular weight allergenic protein for further studies on cross-reactivity and development of diagnostic and therapeutic approaches. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Medizin | en_US |
dc.relation.ispartofseries | Allergo Journal International, Vol. 23: p. 233–239 | |
dc.subject | Ash pollen | en_US |
dc.subject | Allergen | en_US |
dc.subject | 2D electrophoresis | en_US |
dc.subject | IgE immunoblotting | en_US |
dc.subject | Calreticulin | en_US |
dc.title | Identification of a 53kDa protein, as a new high molecular weight allergen from fraxinusexcelsior (Ash) pollen | en_US |
dc.type | Article | en_US |
item.languageiso639-1 | en | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
sebata.pdf | Abstract | 123.55 kB | Adobe PDF | View/Open |
Page view(s)
22
checked on Aug 30, 2024
Download(s)
6
checked on Aug 30, 2024
Google ScholarTM
Check
Items in MSUIR are protected by copyright, with all rights reserved, unless otherwise indicated.