Please use this identifier to cite or link to this item: https://cris.library.msu.ac.zw//handle/11408/6087
Title: Plasma metabolite profiling for S. haematobium biomarkers of infection in pre-school aged children in Shamva District, Zimbabwe
Authors: Herald Midzi
Thajasvarie Naicker
Arthur Vengesai
Emilia T. Choto
Petros Muchesa
Maritha Kasambala
Tariro L. Mduluza-Jokonya
Victor Muleya
Elliot Nyagumbo
Donald Tafirenyika Kapanga
Lucy Mabaya
Francisca Mutapi
Takafira Mduluza
Department of Biotechnology and Biochemistry, University of Zimbabwe, Harare, Zimbabwe; Optics and Imaging, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, KwaZulu-Natal, Durban, South Africa
Optics and Imaging, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, KwaZulu-Natal, Durban, South Africa
Department of Biochemistry, Faculty of Medicine and Health Sciences, Midlands State University, Gweru, Zimbabwe
School of Biomedical Sciences, Great Zimbabwe University, Masvingo, Zimbabwe
Water and Health Research Centre, University of Johannesburg, Johannesburg, South Africa
Department of Biological Sciences and Ecology, University of Zimbabwe, Harare, Zimbabwe
Optics and Imaging, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, KwaZulu-Natal, Durban, South Africa; Faculty of Medicine and Health Science, University of Zimbabwe, Harare, Zimbabwe
Department of Biochemistry, Faculty of Medicine and Health Sciences, Midlands State University, Gweru, Zimbabwe
National Pathology Research and Diagnostic Centre, Midlands State University, Gweru, Zimbabwe
National Pathology Research and Diagnostic Centre, Midlands State University, Gweru, Zimbabwe
National Pathology Research and Diagnostic Centre, Midlands State University, Gweru, Zimbabwe
Institute of Immunology and Infection Research, University of Edinburgh, Ashworth Laboratories, Edinburgh, United Kingdom
Department of Biotechnology and Biochemistry, University of Zimbabwe, Harare, Zimbabwe; Optics and Imaging, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, KwaZulu-Natal, Durban, South Africa
Keywords: metabolites
plasma
biomarkers
S. haematobium
Pre-school aged children
Issue Date: 6-Mar-2024
Publisher: Frontiers Media
Abstract: Background: Metabolomics approaches are indispensable tools in infection biomarker discovery efforts as they shed light on the underlying pathophysiological mechanisms of disease. In this study, we analysed plasma metabolites that can be used as biomarkers of urogenital schistosomiasis in pre-school aged children below the age of five. Methods: A case-control study was conducted involving 82 pre-school aged children that were age- and sex-matched. Urine samples were collected for three consecutive days to detect S. haematobium infection using urine filtration. Blood samples were also collected and processed to obtain plasma. Beckman Coulter AU480 chemistry analyser and commercial metabolite kits were used for profiling biomarkers in plasma samples. Descriptive statistics and MetaboAnalyst tool, were used for metabolite analysis. For the determination of diagnostic efficiency of plasma biomarkers, the area under the curve (AUC) was calculated from receiver operating characteristic curves at 95% CI. Results: Succinic acid, glucose-6-phosphate, phosphatidylcholine, alanine and creatinine levels in plasma were significantly associated with urogenital schistosomiasis (p<0.005) at the population level. Significant increase in concentration at 1.5-fold change (FC) threshold was highest for glucose-6-phosphate with FC value of 2.02 followed by creatinine, albumin and phosphatidylcholine. Creatinine was significantly downregulated with a FC value of 1.98. Of the six dysregulated metabolic pathways, glucose and sucrose metabolism were predominantly affected. Glucose-6-phosphate had the highest AUC (0.81), sensitivity (88.85%) and specificity (90.37%). Phosphatidylcholine and succinic acid also had AUC values greater than 0.7. Conclusion: Urogenital schistosomiasis affects the energy-related metabolic pathways in pre-school aged children. Glucose-6-phosphate was identified as a potential indicator of infection at the population level. Furthermore, we recommend intensive validation of schistosome metabolite biomarkers.
URI: https://cris.library.msu.ac.zw//handle/11408/6087
Appears in Collections:Research Papers

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