Context-dependent integrated stress resistance promotes a global invasive pest

Abstract

In nature, insects concurrently face multiple environmental stressors, a sce- nario likely increasing with climate change. Integrated stress resistance (ISR) thus often improves fitness and could drive invasiveness, but how physiological mechanisms influ- ence invasion has lacked examination. Here, we investigated cross-tolerance to abiotic stress factors which may influence range limits in the South American tomato pinworm— a global invader that is an ecologically and socially damaging crop pest. Specifically, we tested the effects of prior rapid cold- and heat-hardening (RCH and RHH), fasting, and desiccation on cold and heat tolerance traits, as well as starvation and desiccation sur- vivability between T. absoluta life stages. Acclimation effects on critical thermal minima (CT min ) and maxima (CT max ) were inconsistent, showing significantly deleterious effects of RCH on adult CT max and CT min and, conversely, beneficial acclimation effects of RCH on larval CT min . While no beneficial effects of desiccation acclimation were recorded for desiccation tolerance, fasted individuals had significantly higher survival in adults, whereas fasting negatively affected larval tolerances. Furthermore, fasted and desiccation acclimated adults had significantly higher starvation tolerance, showing strong evidence for cross-tolerance. Our results show context-dependent ISR traits that may promote T. absoluta fitness and competitiveness. Given the frequent overlapping occurrence of these divergent stressors, ISR reported here may thus partly elucidate the observed rapid global spread of T. absoluta into more stressful environments than expected. This information is vital in determining the underpinnings of multistressor responses, which are fundamental in forecasting species responses to changing environments and management responses.