Please use this identifier to cite or link to this item: https://cris.library.msu.ac.zw//handle/11408/4929
Title: In Silico Study of Cucurbita maxima Compounds as Potential Therapeutics Against Schistosomiasis
Authors: Mtemeli, Floryn Lynorah
Shoko, Ryman
Ndlovu, Joice
Mugumbate, Grace
Keywords: Schistosomiasis
Cucurbita maxima
purine nucleoside phosphorylase
28-kDa glutathione S-transferase
pharmacokinetics
Issue Date: 2022
Publisher: SAGE Publications
Series/Report no.: Bioinformatics and Biology Insights;16, 11779322221100741
Abstract: Schistosomiasis, a disease usually related to poverty and poor sanitation, affects more than 200 million people worldwide. Since the 1970s, the medical sector has depended on a single drug, praziquantel, for the treatment of the disease. The emerging evidence of resistance of the Schistosoma parasite to praziquantel and the drug’s inefficacy against juvenile stages of the parasite makes the need to find alternative drugs an urgent matter. In this study, we explored the inhibition potential of compounds from Cucurbita maxima using molecular docking studies on Schistosoma mansoni purine nucleoside phosphorylase (SmPNP) and Schistosoma haematobium 28-kDa glutathione S-transferase (Sh28kDaGST). Following molecular docking studies and analysis of the active sites, the primary amino acids that were observed and shown to be involved in the SmPNP-ligand interaction are CYS 33, ARG 86, HIS 88, TYR 90, ALA 118, ALA 119, PRO 200, TYR 202, GLU 203, VAL 219, MET 221, THR 244, ASN 245, PRO 257 and HIS 259. For the Sh28dKa-ligand interaction, the primary amino acids were PHE 11, ARG 16, TRP 41, LEU 53, GLU 70 and SER 71. Momordicoside I aglycone binds to SmPNP with the lowest binding affinity of -7.9 kcal/mol by pi sigma bond interactions with HIS 88. Balsaminoside B binds to Sh28kDaGST with a binding affinity of −7.6 kcal/mol by hydrogen bond interaction with TRP 41, LEU 53 and SER 71. Pharmacokinetic studies showed favourable drug-like properties for the 10 compounds that exhibited the lowest binding energies. Therefore, we propose that bioactive compounds from C. maxima be considered as potential novel drug hits in the treatment of schistosomiasis.
URI: https://doi.org/10.1177/11779322221100741
http://hdl.handle.net/11408/4929
ISSN: 1177-9322
Appears in Collections:Research Papers

Files in This Item:
File Description SizeFormat 
In Silico Study of Cucurbita maxima Compounds as P.pdfAbstract104.11 kBAdobe PDFView/Open
Show full item record

Page view(s)

20
checked on Mar 28, 2024

Google ScholarTM

Check


Items in MSUIR are protected by copyright, with all rights reserved, unless otherwise indicated.